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Journal: Brazilian Journal of Microbiology
Article Title: A novel Trichoderma reesei mutant RP698 with enhanced cellulase production
doi: 10.1007/s42770-019-00167-2
Figure Lengend Snippet: a Growth of T. reesei QM9414 and T. reesei RP698 on PDA slants after 4 days at 27 °C. b Time course of cellulase production by T. reesei RP698. Culture conditions: Mandels’ medium containing Avicel 1% (w/v), 27 °C, pH 6.0, and 110 rpm. The black-filled square represents the Avicelase activity (100% is 0.78 ± 0.04 U/mL). The red-filled circle represents the CMCase activity (100% is 11.8 ± 0.92 U/mL). The blue up-pointing arrow represents the FPase activity (100% is 0.72 ± 0.06 U/mL). All data are the means ± SD of three experiments (n = 3)
Article Snippet: Random mutagenesis by UV light Ten milliliters of spore suspension (10 7 spores/mL) from
Techniques: Activity Assay
Journal: Brazilian Journal of Microbiology
Article Title: A novel Trichoderma reesei mutant RP698 with enhanced cellulase production
doi: 10.1007/s42770-019-00167-2
Figure Lengend Snippet: a Disintegration of Whatman No. 1 filter paper after 1 h, at 50 °C, pH 5.0, with crude cellulases (10 FPU/g of substrate) from T. reesei QM9414 and T. reesei RP698. The final enzymatic assay volume was 5 mL, and the first and third tubes are control assays with crude cellulase previously inactivated by heating. b Influence of glucose as carbon source on the production of cellulases by T. reesei RP698. One hundred percent of the activities are Avicelase 0.46 ± 0.03 U/mL; CMCase 11.60 ± 0.92 U/mL; FPase 0.43 ± 0.03 U/mL. c pH optima and d temperature optima of cellulase activity from T. reesei RP698. Symbols: The black-filled square represents the Avicelase activity (100% was 0.81 ± 0.05 U/mL). The red-filled circle represents the CMCase activity (100% was 12.52 ± 1.3 U/mL). The blue up-pointing arrow represents the FPase activity (100% was 0.78 ± 0.06 U/mL). e Thermal stability of CMCase activity from T. reesei RP698. The crude filtrate was heated at 40 °C (black-filled square), 50 °C (red-filled circle), 60 °C (blue up-pointing arrow), and 70 °C (orange down-pointing arrow) without substrate and tested for residual CMCase activity. One hundred percent of CMCase activity was 12.30 ± 0.98 U/mL. All data are the means ± SD of three experiments (n = 3)
Article Snippet: Random mutagenesis by UV light Ten milliliters of spore suspension (10 7 spores/mL) from
Techniques: Enzymatic Assay, Control, Activity Assay
Journal: Brazilian Journal of Microbiology
Article Title: A novel Trichoderma reesei mutant RP698 with enhanced cellulase production
doi: 10.1007/s42770-019-00167-2
Figure Lengend Snippet: Effect of the addition of glucose (black-filled square) and cellobiose (blue up-pointing arrow) on cellulase activity of crude filtrate from T. reesei RP698 (a) and T. reesei QM9414 (b). The assay was performed using Cellulose-Azure® as substrate, at 60 °C, pH 5.0 for 1 h. One hundred percent of cellulose-azure activity was 0.6 ± 0.09 U/mL. Reducing sugars (c) and glucose (d) released in the filter paper assay by enzymatic mixtures. All the enzymes were applied at 10 FPU/g of substrate and assayed at 50 °C. Symbols (for c and d): The blue up-pointing arrow represents the T. reesei RP698 cellulase extract. The red-filled circle represents the T. reesei RP698 cellulase extract plus crude StGTBG. The black-filled square represents the T. reesei RP698 cellulase extract plus crude StGSBG. All data are the means ± SD of three experiments (n = 3)
Article Snippet: Random mutagenesis by UV light Ten milliliters of spore suspension (10 7 spores/mL) from
Techniques: Activity Assay, Paper Assay